BROADCASTS.com

b'dessan\\u3092\\u30b2\\u30b9\\u30c8\\u306b\\u8fce\\u3048\\u3001CRISPR\\u306e\\u4ed5\\u7d44\\u307f\\u3092\\u5229\\u7528\\u3057\\u305f\\u69d8\\u3005\\u306a\\u6280\\u8853\\u3084\\u907a\\u4f1d\\u5b50\\u56de\\u8def\\u3001\\u3053\\u308c\\u304b\\u3089\\u306e\\u767a\\u5c55\\u306b\\u3064\\u3044\\u3066\\u8a71\\u3057\\u307e\\u3057\\u305f\\u3002

Show notes

\\n
• The Nobel Prize in Chemistry 2020\\u2026The Nobel Prize in Chemistry 2020 was awarded jointly to Emmanuelle Charpentier and Jennifer A. Doudna \\u201cfor the development of a method for genome editing.\\u201d
\\n
• Scientifc Background on the Nobel Prize in Chemistry 2020 A TOOL FOR GENOME EDITING\\u2026\\u30ce\\u30fc\\u30d9\\u30eb\\u8ca1\\u56e3\\u306b\\u3088\\u308b\\u8a73\\u7d30\\u306aCRISPR\\u7814\\u7a76\\u306e\\u30ec\\u30d3\\u30e5\\u30fc\\u3001\\u305d\\u3057\\u3066\\u306a\\u305cDoudna\\u3068Charpentier\\u306e\\u4e8c\\u4eba\\u304c\\u53d7\\u8cde\\u306b\\u5024\\u3059\\u308b\\u306e\\u304b\\u306b\\u3064\\u3044\\u3066\\u8aac\\u660e\\u3057\\u3066\\u3044\\u308b\\u3002
\\n
• 76. The Chimeric RNA, Researchat.fm\\u2026\\u30b2\\u30ce\\u30e0\\u7de8\\u96c6\\u306b\\u3064\\u3044\\u3066dessan\\u3092\\u30b2\\u30b9\\u30c8\\u306b\\u8fce\\u3048\\u3066\\u8a71\\u3057\\u307e\\u3057\\u305f\\u3002
\\n
• A Programmable Dual-RNA\\u2013Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science 2012\\u2026Charpentier\\u3068Doudna\\u306b\\u3088\\u308b\\u30ce\\u30fc\\u30d9\\u30eb\\u8cde\\u306b\\u3064\\u306a\\u304c\\u308b\\u8ad6\\u6587\\u306e\\u4e00\\u3064\\u3002CRISPR\\u2013Cas9\\u30b7\\u30b9\\u30c6\\u30e0\\u304c\\u3053\\u306e\\u8ad6\\u6587\\u306b\\u3088\\u3063\\u3066\\u305d\\u306e\\u5927\\u67a0\\u304c\\u660e\\u3089\\u304b\\u306b\\u3055\\u308c\\u305f\\u3002
\\n
• Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 2013\\u2026Feng Zhang lab\\u306b\\u3088\\u308b\\u30d2\\u30c8\\u7d30\\u80de\\u306b\\u304a\\u3051\\u308b\\u30b2\\u30ce\\u30e0\\u7de8\\u96c6\\u6280\\u8853\\u306e\\u5831\\u544a\\u3002
\\n
• RNA-Guided Human Genome Engineering via Cas9. Science 2012\\u2026George Church lab\\u306b\\u3088\\u308b\\u30d2\\u30c8\\u7d30\\u80de\\u306b\\u304a\\u3051\\u308b\\u30b2\\u30ce\\u30e0\\u7de8\\u96c6\\u6280\\u8853\\u306e\\u5831\\u544a\\u3082\\u540c\\u6642\\u306b\\u63b2\\u8f09\\u3055\\u308c\\u305f\\u3002
\\n
• First rounders: Feng Zhang (Podcast)\\u2026Feng Zhang\\u304c\\u51fa\\u6f14\\u3057\\u305fNatute Biotechnology\\u306e\\u30dd\\u30c3\\u30c9\\u30ad\\u30e3\\u30b9\\u30c8\\u3002\\u304a\\u3059\\u3059\\u3081\\u3067\\u3059\\u3002
\\n
• 26. Cool tech googlability, Researchat.fm\\u2026RNA\\u3092\\u6a19\\u7684\\u306b\\u3067\\u304d\\u308bCas13b\\u306b\\u3064\\u3044\\u3066\\u306f\\u3001\\u30a8\\u30d4\\u30bd\\u30fc\\u30c926\\u3067\\u7d39\\u4ecb\\u3057\\u307e\\u3057\\u305f\\u3002
\\n
• Cas14 (crisp_bio)\\u2026\\u201cCas14\\u306f\\u3001PAM\\u306b\\u4f9d\\u5b58\\u3057\\u306a\\u3044ssDNA\\u5207\\u65ad\\u6d3b\\u6027\\u306b\\u52a0\\u3048\\u3066\\u3001PAM\\u306b\\u4f9d\\u5b58\\u3059\\u308bdsDNA\\u5207\\u65ad\\u6d3b\\u6027\\u3082\\u5e2f\\u3073\\u3066\\u3044\\u308b\\u201d
\\n
• CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature 2019\\u2026CasX
\\n
• Transposon-encoded CRISPR\\u2013Cas systems direct RNA-guided DNA integration. Nature 2019\\u2026\\u30c8\\u30e9\\u30f3\\u30b9\\u30dd\\u30be\\u30f3\\u578b\\u306eCas\\u30b7\\u30b9\\u30c6\\u30e0\\u306e\\u5831\\u544a\\u3002
\\n
• RNA-programmed genome editing in human cells. eLife 2013\\u2026Doudna lab\\u306b\\u3088\\u308b\\u30d2\\u30c8\\u7d30\\u80de\\u306b\\u304a\\u3051\\u308b\\u30b2\\u30ce\\u30e0\\u7de8\\u96c6\\u6280\\u8853\\u306e\\u5831\\u544a\\u3002Feng\\u3084Church\\u3089\\u3088\\u308a\\u3082\\u5c11\\u3057\\u3060\\u3051\\u9045\\u304b\\u3063\\u305f\\u3002
\\n
• Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9. Nature Communications 2014
\\n
• Ep52. Split into a row
\\n
• Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity. Cell 2013\\u2026Double nicking (2\\u3064\\u306egRNA\\u3068Cas9 nickase)\\u306b\\u3088\\u308b\\u3088\\u308a\\u6b63\\u78ba\\u306a\\u30b2\\u30ce\\u30e0\\u7de8\\u96c6\\u65b9\\u6cd5\\u304c\\u793a\\u3055\\u308c\\u305f\\u3002
\\n
• Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature 2015\\u2026Cas9\\u3092\\u7528\\u3044\\u305f\\u8ee2\\u5199\\u306e\\u6d3b\\u6027\\u5316\\u624b\\u6cd5\\u3002
\\n
• Live visualization of chromatin dynamics with fluorescent TALEs. Nature Structural & Molecular Biology 2013 \\u2026 TALEN\\u3092\\u7528\\u3044\\u305f\\u67d3\\u8272\\u4f53\\u306e\\u7279\\u5b9a\\u9818\\u57df\\u306e\\u30a4\\u30e1\\u30fc\\u30b8\\u30f3\\u30b0\\u65b9\\u6cd5
\\n
• Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell 2013\\u2026dCas9-EGFP\\u306b\\u3088\\u308b\\u751f\\u7d30\\u80de\\u306e\\u30a4\\u30e1\\u30fc\\u30b8\\u30f3\\u30b0\\u6280\\u8853\\u3002SpCas9\\u306e\\u5834\\u5408\\u306f\\u3001D10A\\u3068H840A\\u306e\\uff12\\u3064\\u306e\\u5909\\u7570\\u3092\\u5165\\u308c\\u308b\\u3053\\u3068\\u3067\\u3001DNA\\u306b\\u7d50\\u5408\\u3059\\u308b\\u304c\\u5207\\u65ad\\u3057\\u306a\\u3044dead Cas9 (dCas9)\\u3068\\u3057\\u3066\\u5229\\u7528\\u3059\\u308b\\u3053\\u3068\\u304c\\u3067\\u304d\\u308b\\u3002
\\n
• Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9. Nature Communications 2017\\u2026\\u30ac\\u30a4\\u30c9RNA\\u306bMS2 loop\\u3092\\u305f\\u304f\\u3055\\u3093\\u3064\\u306a\\u3052\\u308b\\u3053\\u3068\\u3067 (14\\u500b!)\\u3001\\u660e\\u308b\\u3044\\u8f1d\\u70b9\\u3092\\u5f97\\u308b\\u3053\\u3068\\u304c\\u3067\\u304d\\u308b\\u3002
\\n
• CRISPR-mediated live imaging of genome editing and transcription. Science 2019\\u2026\\u3053\\u3061\\u3089\\u306f\\u86cd\\u5149\\u6a19\\u8b58\\u3057\\u305f\\u30ac\\u30a4\\u30c9RNA\\u3092\\u5229\\u7528\\u3057\\u305f\\u751f\\u7d30\\u80de\\u30a4\\u30e1\\u30fc\\u30b8\\u30f3\\u30b0\\u65b9\\u6cd5\\u3002
\\n
• A protein tagging system for signal amplification in gene expression and fluorescence imaging. Cell 2014\\u2026Sun tag\\u3068Cas9\\u3092\\u7528\\u3044\\u305f\\u30a4\\u30e1\\u30fc\\u30b8\\u30f3\\u30b0\\u65b9\\u6cd5\\u3002
\\n
• Split Green Fluorescent Proteins: Scope, Limitations, and Outlook\\u2026Split GFP
\\n
• Programmable RNA tracking in Live Cells with CRISPR/Cas9. Cell 2016\\u2026PAMmer\\u306b\\u3088\\u308bSpCas9\\u306emRNA\\u30a4\\u30e1\\u30fc\\u30b8\\u30f3\\u30b0
\\n
• CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear Organization. Cell 2018 \\u2026 CRISPR-GO\\uff1aCRISPR\\u6280\\u8853\\u3001\\u6838\\u5185\\u3067\\u306e\\u30b2\\u30ce\\u30e0\\u7a7a\\u9593\\u69cb\\u9020\\u3001\\u30dd\\u30c3\\u30c9\\u30ad\\u30e3\\u30b9\\u30c8\\u5185\\u3067\\u306f\\u30b2\\u30ce\\u30e0\\u540c\\u58eb\\u3092\\u5bc4\\u305b\\u308b\\u3068\\u3044\\u3046\\u8aac\\u660e\\u3092\\u3057\\u3066\\u3044\\u307e\\u3057\\u305f\\u304c\\u3001\\u4eca\\u8abf\\u3079\\u3066\\u307f\\u308b\\u3068\\u7279\\u5b9a\\u306e\\u30b2\\u30ce\\u30e0\\u9818\\u57df\\u3068\\u6838\\u819c\\u3084\\u30ab\\u30cf\\u30fc\\u30eb\\u4f53\\u3078\\u306e\\u518d\\u914d\\u7f6e\\u3068\\u3044\\u3046\\u3053\\u3068\\u3067\\u3057\\u305f\\u3002
\\n
• Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping. Nature Communications 2017 \\u2026 \\u3053\\u3061\\u3089\\u304cCRISPR\\u306e\\u4ed5\\u7d44\\u307f\\u3092\\u7528\\u3044\\u308b\\u3053\\u3068\\u3067\\u4eba\\u5de5\\u7684\\u306b\\u67d3\\u8272\\u4f53\\u5185\\u90e8\\u306b\\u30eb\\u30fc\\u30d7\\u3092\\u4f5c\\u6210\\u3057\\u305f\\u8ad6\\u6587\\u3002
\\n
• Transcriptional repression mediated by repositioning of genes to the nuclear lamina. Nature 2008 \\u2026 LacO-LacI\\u306e\\u4ed5\\u7d44\\u307f\\u3092\\u7528\\u3044\\u308b\\u3053\\u3068\\u3067\\u30b2\\u30ce\\u30e0\\u306e\\u7279\\u5b9a\\u9818\\u57df\\u306bLacO array\\u3092\\u5dee\\u3057\\u8fbc\\u307f\\u3001\\u6838\\u819c\\u306b\\u5c40\\u5728\\u3055\\u305b\\u305fLacI\\u306b\\u7d50\\u5408\\u3055\\u305b\\u308b\\u3053\\u3068\\u3067\\u3042\\u308b\\u907a\\u4f1d\\u5b50\\u9818\\u57df\\u3092\\u6838\\u819c\\u5074\\u306b\\u8a98\\u5c0e\\u3057\\u3088\\u3046\\u3068\\u3057\\u305f\\u8ad6\\u6587\\u3002\\u6700\\u521d\\u306b\\u3053\\u306e\\u8ad6\\u6587\\u3092\\u8aad\\u3093\\u3060\\u6642\\u306f\\u305d\\u306e\\u30a2\\u30a4\\u30c7\\u30a2\\u306b\\u305f\\u307e\\u3052\\u307e\\u3057\\u305f\\u3002
\\n
• 9. One-shot beautiful experiment (Researchat.fm)\\u2026\\u4eba\\u5de5\\u7684\\u306aDNA\\u9818\\u57df\\u3078\\u7d30\\u80de\\u5185\\u306e\\u60c5\\u5831\\uff08\\u7d30\\u80de\\u7cfb\\u8b5c\\uff09\\u3092\\u66f8\\u304d\\u8fbc\\u3080\\u6280\\u8853\\u306b\\u3064\\u3044\\u3066\\u30a8\\u30d4\\u30bd\\u30fc\\u30c99\\u3067\\u8a71\\u3057\\u307e\\u3057\\u305f\\u3002
\\n
• CRISPR\\u2013Cas encoding of a digital movie into the genomes of a population of living bacteria. Nature 2017\\u2026George Church\\u3089\\u306f\\u3001Cas1-Cas2\\u30b7\\u30b9\\u30c6\\u30e0\\u306b\\u3088\\u3063\\u3066\\u99ac\\u306e\\u52d5\\u753b\\u3092\\u30d0\\u30af\\u30c6\\u30ea\\u30a2\\u30b2\\u30ce\\u30e0\\u66f8\\u304d\\u8fbc\\u307f\\u3001\\u305d\\u308c\\u3092\\u8aad\\u307f\\u51fa\\u3059\\u3053\\u3068\\u306b\\u6210\\u529f\\u3057\\u305f\\u3002
\\n
• Multiplex recording of cellular events over time on CRISPR biological tape. Science 2017\\u2026\\u30b3\\u30d4\\u30fc\\u6570\\u306e\\u7570\\u306a\\u308b2\\u3064\\u306e\\u30d7\\u30e9\\u30b9\\u30df\\u30c9\\u3092Cas1-Cas2\\u3067\\u53d6\\u308a\\u8fbc\\u307e\\u305b\\u3066\\u3001\\u7d30\\u80de\\u5185\\u3067\\u4eba\\u5de5\\u7684\\u306a\\u6642\\u8a08\\u306e\\u3088\\u3046\\u306a\\u4ed5\\u7d44\\u307f\\u3092\\u5b9f\\u73fe\\u3057\\u305f\\u3002
\\n
• Single-Nucleotide-Resolution Computing and Memory in Living Cells. Molecular Cell 2019\\u2026Tim Liu Lab\\u306b\\u3088\\u308b\\u8907\\u96d1\\u306a\\u907a\\u4f1d\\u5b50\\u56de\\u8def\\u306e\\u5b9f\\u73fe\\u3002DOMINO\\u306b\\u3064\\u3044\\u3066\\u306f\\u3001\\u30d7\\u30ed\\u30e2\\u30fc\\u30bf\\u30fc\\u914d\\u5217\\u3092\\u6a19\\u7684\\u306b\\u3057\\u3066\\u3044\\u308b\\u306e\\u3067\\u306f\\u306a\\u304f\\u30aa\\u30da\\u30ec\\u30fc\\u30bf\\u30fc\\u914d\\u5217\\u3067\\u3057\\u305f\\u3002
\\n
• Rewritable multi-event analog recording in bacterial and mammalian cells. Science 2018\\u2026David Liu lab\\u304b\\u3089\\u5831\\u544a\\u3055\\u308c\\u305f\\u30ac\\u30a4\\u30c9RNA\\u306b\\u3088\\u3063\\u3066\\u9023\\u9396\\u3059\\u308b\\u907a\\u4f1d\\u5b50\\u56de\\u8def\\uff08\\u30ab\\u30b9\\u30b1\\u30fc\\u30c9\\uff09\\u306e\\u5b9f\\u73fe\\u3002
\\n
• Terminal Deoxynucleotidyl Transferase, TdT\\u2026\\u30c6\\u30f3\\u30d7\\u30ec\\u30fc\\u30c8\\u306b\\u4f9d\\u5b58\\u3057\\u306a\\u3044DNA\\u5408\\u6210\\u3092\\u53ef\\u80fd\\u306b\\u3059\\u308b\\u9175\\u7d20\\u3002
\\n
• Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nature Biotechnology 2012\\u2026GFP Timer
\\n
• Permanent genetic memory with >1-byte capacity. Nature Methods 2014
\\n
• Continuous genetic recording with self-targeting CRISPR-Cas in human cells. Science 2016\\u2026\\u81ea\\u5206\\u3067\\u81ea\\u5206\\u306e\\u30ac\\u30a4\\u30c9RNA\\u3092\\u7de8\\u96c6\\u3059\\u308b\\u3053\\u3068\\u3067\\u3001\\u7406\\u8ad6\\u7684\\u306b\\u306f\\u7121\\u9650\\u306b\\u60c5\\u5831\\u3092\\u66f8\\u304d\\u8fbc\\u3080\\u65b9\\u6cd5\\u304c\\u63d0\\u6848\\u3055\\u308c\\u305f\\u304c\\u3001\\u9818\\u57df\\u304c\\u58ca\\u308c\\u3066\\u3057\\u307e\\u3046\\u554f\\u984c\\u3082\\u3042\\u308b\\u3002
\\n
• Ten Simple Rules to Win a Nobel Prize.
\\n
• \\u30d8\\u30f3\\u30ea\\u30fc\\u30fb\\u30d6\\u30e9\\u30c3\\u30b0 (Wikipedia)
\\n
• iPS\\u7d30\\u80de (Wikipedia)
\\n
• \\u56fd\\u5883\\u306a\\u304d\\u533b\\u5e2b\\u56e3
\\n
• Human Genome Project
\\n
• Xiaowei Zhuang
\\n
• Expansion microscopy (Wikipedia)
\\n
• Renato Dulbecco (Wikipedia)
\\n
• Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs. Nature Biotechnology 2019\\u2026LEAPER
\\n
• crisp_bio \\u2026 \\u4e16\\u754c\\u5e83\\u3057\\u3068\\u3044\\u3048\\u3067\\u3082\\u3001\\u3053\\u308c\\u3060\\u3051CRISPR\\u306e\\u6700\\u65b0\\u60c5\\u5831\\u304c\\u307e\\u3068\\u307e\\u3063\\u3066\\u3044\\u308b\\u30b5\\u30a4\\u30c8\\u306fCRISP_BIO\\u3055\\u3093\\u306e\\u4ed6\\u306b\\u4e16\\u306e\\u4e2d\\u306b\\u306f\\u5b58\\u5728\\u3057\\u307e\\u305b\\u3093\\u3002\\u65e5\\u672c\\u8a9e\\u3067CRISPR\\u306e\\u6700\\u5148\\u7aef\\u60c5\\u5831\\u3092\\u8ffd\\u3048\\u308b\\u559c\\u3073\\u3002CRISP_BIO\\u3055\\u3093\\u3001\\u3044\\u3064\\u3082\\u3042\\u308a\\u304c\\u3068\\u3046\\u3054\\u3056\\u3044\\u307e\\u3059\\u3002
\\n

Editorial notes

\\n
• 1\\u5206\\u3067\\u308f\\u304b\\u308b\\u3068\\u304b\\u7121\\u7406\\u306a\\u306e\\u3067\\u3059\\u304c\\u3001\\u4e00\\u65b9\\u3067\\u8a00\\u8449\\u3092\\u5c3d\\u304f\\u305b\\u3070\\u308f\\u304b\\u308b\\u53ef\\u80fd\\u6027\\u306b\\u3064\\u3044\\u3066\\u3082\\u540c\\u6642\\u306b\\u4fe1\\u3058\\u3066\\u304a\\u308a\\u307e\\u3059\\u306e\\u3067\\u2026(soh)
\\n
• \\u601d\\u3044\\u51fa\\u3057\\u306a\\u304c\\u3089\\u3069\\u3093\\u3069\\u3093\\u8a71\\u3057\\u3066\\u3044\\u308b\\u306e\\u3067\\u3001\\u5f8c\\u304b\\u3089\\u805e\\u304d\\u8fd4\\u3059\\u3068\\u7d30\\u90e8\\u304c\\u9593\\u9055\\u3063\\u3066\\u3044\\u305f\\u308a\\u3057\\u3066\\u3044\\u307e\\u3059\\u3002\\u6c17\\u306b\\u306a\\u3063\\u305f\\u65b9\\u306fshow notes\\u3092\\u3054\\u53c2\\u7167\\u304f\\u3060\\u3055\\u3044\\u3002(dessan)
\\n
• \\u3044\\u3044\\u611f\\u3058\\u306e\\u30b0\\u30eb\\u30fc\\u30f4\\u304c\\u307f\\u3089\\u308c\\u3066\\u3088\\u304b\\u3063\\u305f\\u3067\\u3059\\u3002\\u30dd\\u30c3\\u30c9\\u30ad\\u30e3\\u30b9\\u30c8\\u3084\\u3063\\u3066\\u304d\\u3066\\u3088\\u304b\\u3063\\u305f\\u3067\\u3059\\u3002(tadasu)
\\n
• \\u6700\\u521d\\u306b\\u558b\\u3089\\u3093\\u3068\\u51fa\\u756a\\u304c\\u7121\\u304f\\u306a\\u308b\\uff01\\u3068\\u601d\\u3063\\u3066\\u3053\\u308c\\u307e\\u3067\\u306e\\u6d41\\u308c\\u3092\\u307e\\u3068\\u3081\\u3066\\u307f\\u305f\\u3093\\u3067\\u3059\\u304c\\u7d30\\u304b\\u304f\\u8272\\u3005\\u30df\\u30b9\\u3063\\u3066\\u307e\\u3057\\u305f\\u2026(coela)
\\n