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b'In this episode of the Epigenetics Podcast, we caught up with Jop Kind from Hubrecht Institute to talk about his work on single cell DamID, EpiDamID, and Lamina Associated Domains (LADs).\\nJop Kind started out developing single cell DamID (scDamID), based on the DamID technique. First, this technique was adapted to a microscopic readout which enabled them to follow the localisation of chromatin domains after cell division. Next, the lab expanded this technique into the NGS space and created genome-wide maps of nuclear lamina Interactions in single human cells. Since LADs are in a heterochromatic chromatin context, the lab expanded scDamID into the epigenetic space. They first combined it with a transcriptional readout.\\xa0 Later-on they developed EpiDamID, a method to target a diverse set of chromatin types by taking advantage of the binding specificities of single-chain variable fragment antibodies, engineered chromatin reader domains, and endogenous chromatin-binding proteins.\\n\\xa0\\nReferences\\n\\nKind, J., Pagie, L., Ortabozkoyun, H., Boyle, S., de Vries, S. S., Janssen, H., Amendola, M., Nolen, L. D., Bickmore, W. A., & van Steensel, B. (2013). Single-Cell Dynamics of Genome-Nuclear Lamina Interactions. Cell, 153(1), 178\\u2013192. https://doi.org/10.1016/j.cell.2013.02.028\\n\\n\\nKind, J., Pagie, L., de Vries, S. S., Nahidiazar, L., Dey, S. S., Bienko, M., Zhan, Y., Lajoie, B., de Graaf, C. A., Amendola, M., Fudenberg, G., Imakaev, M., Mirny, L. A., Jalink, K., Dekker, J., van Oudenaarden, A., & van Steensel, B. (2015). Genome-wide Maps of Nuclear Lamina Interactions in Single Human Cells. Cell, 163(1), 134\\u2013147. https://doi.org/10.1016/j.cell.2015.08.040\\n\\n\\nBorsos, M., Perricone, S.M., Schauer, T. et al. Genome\\u2013lamina interactions are established de novo in the early mouse embryo. Nature 569, 729\\u2013733 (2019). https://doi.org/10.1038/s41586-019-1233-0\\n\\n\\nMarkodimitraki, C. M., Rang, F. J., Rooijers, K., de Vries, S. S., Chialastri, A., de Luca, K. L., Lochs, S. J. A., Mooijman, D., Dey, S. S., & Kind, J. (2020). Simultaneous quantification of protein\\u2013DNA interactions and transcriptomes in single cells with scDam&T-seq. Nature Protocols, 15(6), 1922\\u20131953. https://doi.org/10.1038/s41596-020-0314-8\\n\\n\\nRang, F. J., de Luca, K. L., de Vries, S. S., Valdes-Quezada, C., Boele, E., Nguyen, P. D., Guerreiro, I., Sato, Y., Kimura, H., Bakkers, J., & Kind, J. (2022). Single-cell profiling of transcriptome and histone modifications with EpiDamID. Molecular Cell, 82(10), 1956-1970.e14. https://doi.org/10.1016/j.molcel.2022.03.009\\n\\xa0\\n\\nRelated Episodes\\n\\nDosage Compensation in Drosophila (Asifa Akhtar)\\n\\n\\nChromatin Profiling: From ChIP to CUT&RUN, CUT&Tag and CUTAC (Steven Henikoff)\\n\\n\\nSingle Cell Epigenomics in Neuronal Development (Tim Petros)\\n\\n\\xa0\\n\\nContact\\n\\nEpigenetics Podcast on Twitter\\n\\n\\nEpigenetics Podcast on Instagram\\n\\n\\nEpigenetics Podcast on Mastodon\\n\\n\\nActive Motif on Twitter\\n\\n\\nActive Motif on LinkedIn\\n\\n\\nEmail: podcast@activemotif.com'