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Callistratus was born in Carthage to Christian parents. In time, he joined the army, where he was the only Christian in his regiment. One of his fellow-soldiers saw Callistratus rise during the night to pray, and reported him to their commander. Callistratus was interrogated, then, when he refused to deny his faith or make sacrifice to the idols, was harshly beaten, then tied in a sack and thrown in the sea. But the sack burst open and Callistratus emerged from the sea unharmed. Seeing this, forty-nine of his fellow-soldiers confessed Christ, and were beaten and thrown into prison with Callistratus. In prison, Callistratus instructed the newly-enlightened Christians in the faith. Finally, all of them were beheaded, according to some sources in 288, according to others in 304.