BROADCASTS.com

In this episode of the Epigenetics Podcast, we caught up with Karim-Jean Armache from New York University - Grossman School of Medicine to talk about his work on the structural analysis of Polycomb Complex Proteins and molecular mechanisms of chromatin modifying enzymes.\nKarim-Jean Armache started his research career with the structural characterization of the 12-subunit RNA Polymerase II. After starting his own lab he used this knowledge in x-ray crystallography and electron microscopy to study how gene silencing complexes like the PRC complex act on chromatin and influence transcription. Further work in the Armache Lab focused on Dot, a \xa0histone H3K79 methyltransferase, and how it acts on chromatin, as well as how it is regulated by Histone-Histone crosstalk.\nReferences\n\nArmache, K. J., Garlick, J. D., Canzio, D., Narlikar, G. J., & Kingston, R. E. (2011). Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 \xc5 resolution. Science (New York, N.Y.), 334(6058), 977\u2013982. https://doi.org/10.1126/science.1210915\n\n\nLee, C. H., Holder, M., Grau, D., Salda\xf1a-Meyer, R., Yu, J. R., Ganai, R. A., Zhang, J., Wang, M., LeRoy, G., Dobenecker, M. W., Reinberg, D., & Armache, K. J. (2018). Distinct Stimulatory Mechanisms Regulate the Catalytic Activity of Polycomb Repressive Complex 2. Molecular cell, 70(3), 435\u2013448.e5. https://doi.org/10.1016/j.molcel.2018.03.019\n\n\nDe Ioannes, P., Leon, V. A., Kuang, Z., Wang, M., Boeke, J. D., Hochwagen, A., & Armache, K. J. (2019). Structure and function of the Orc1 BAH-nucleosome complex. Nature communications, 10(1), 2894. https://doi.org/10.1038/s41467-019-10609-y\n\n\nValencia-S\xe1nchez, M. I., De Ioannes, P., Wang, M., Truong, D. M., Lee, R., Armache, J. P., Boeke, J. D., & Armache, K. J. (2021). Regulation of the Dot1 histone H3K79 methyltransferase by histone H4K16 acetylation. Science (New York, N.Y.), 371(6527), eabc6663. https://doi.org/10.1126/science.abc6663\n\xa0\n\nRelated Episodes\n\nTranscription and Polycomb in Inheritance and Disease (Danny Reinberg)\n\n\nFrom Nucleosome Structure to Function (Karolin Luger)\n\n\nOncohistones as Drivers of Pediatric Brain Tumors (Nada Jabado)\n\n\xa0\nContact\n\nEpigenetics Podcast on Twitter\n\n\nEpigenetics Podcast on Instagram\n\n\nEpigenetics Podcast on Mastodon\n\n\nActive Motif on Twitter\n\n\nActive Motif on LinkedIn\n\n\neMail: podcast@activemotif.com