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In this episode of the Epigenetics Podcast, we caught up with Marcela Sj\xf6berg from the Pontificia Universidad Cat\xf3lica de Chile to talk about her work on the hydroxymethylation landscape in immune cells.\nAt the beginning of her career Marcela Sj\xf6berg worked on Aurora B and Polycomb and how modifications placed by them modulate the binding of RNA Pol II. Later, her focus shifted to examine\xa0cytosine\xa0DNA methylation and hydroxymethylation changes\xa0in immune cells\xa0and\xa0how the epigenetic state of these marks varies\xa0between individuals and\xa0is reprogrammed for Metastable Epialleles in mouse.\xa0More recently, the laboratory is interested on how hydroxymethylation\xa0of transcription factor binding motifs influence binding and activity of the respective transcription factors in\xa0immune cells.\n\xa0\nReferences\n\nSabbattini, P., Sjoberg, M., Nikic, S., Frangini, A., Holmqvist, P.-H., Kunowska, N., Carroll, T., Brookes, E., Arthur, S. J., Pombo, A., & Dillon, N. (2014). An H3K9/S10 methyl-phospho switch modulates Polycomb and Pol II binding at repressed genes during differentiation. Molecular Biology of the Cell, 25(6), 904\u2013915. https://doi.org/10.1091/mbc.e13-10-0628\n\n\nKazachenka, A., Bertozzi, T. M., Sjoberg-Herrera, M. K., Walker, N., Gardner, J., Gunning, R., Pahita, E., Adams, S., Adams, D., & Ferguson-Smith, A. C. (2018). Identification, Characterization, and Heritability of Murine Metastable Epialleles: Implications for Non-genetic Inheritance. Cell, 175(5), 1259-1271.e13. https://doi.org/10.1016/j.cell.2018.09.043\n\n\nWestoby, J., Herrera, M.S., Ferguson-Smith, A.C. et al. Simulation-based benchmarking of isoform quantification in single-cell RNA-seq. Genome Biol 19, 191 (2018). https://doi.org/10.1186/s13059-018-1571-5\n\n\nViner, C., Johnson, J., Walker, N., Shi, H., Sj\xf6berg, M., Adams, D. J., Ferguson-Smith, A. C., Bailey, T. L., & Hoffman, M. M. (2016). Modeling methyl-sensitive transcription factor motifs with an expanded epigenetic alphabet [Preprint]. Bioinformatics. https://doi.org/10.1101/043794\n\xa0\n\nRelated Episodes\n\nThe Role of DNA Methylation in Epilepsy (Katja Kobow)\n\n\nDNA Methylation and Mammalian Development (D\xe9borah Bourc'his)\n\n\nEffects of DNA Methylation on Chromatin Structure and Transcription (Dirk Sch\xfcbeler)\n\n\xa0\nContact\n\nActive Motif on Twitter\n\n\nEpigenetics Podcast on Twitter\n\n\nActive Motif on LinkedIn\n\n\nActive Motif on Facebook\n\n\nEmail: podcast@activemotif.com