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In this episode of the Epigenetics Podcast, we caught up with Charlotte Proudhon from the Institut Curie to talk about her work on circulating tumor DNA and circulating Epi-mutations as biomarkers in cancer.\nCharlotte Proudhon started out her research career by investigating circulating tumor DNA (ctDNA). This kind of DNA is shed into the bloodstream by apoptotic tumor cells and can be analyzed after collection by a simple blood draw, which makes it a very useful biomarker for cancer. Using this approach cancers can be identified by their unique mutational fingerprint. However, soon the limitations of this approach became apparent and the fact that this ctDNA is actually shed into the bloodstream as nucleosomal particles was utilized by the Proudhon team and now the methylation fingerprint of the LINE-1 repeats is used as a biomarker for cancer diagnosis and monitoring of the success of a cancer treatment.\n\xa0\nReferences\n\nDecraene, C., Silveira, A. B., Bidard, F. C., Vall\xe9e, A., Michel, M., Melaabi, S., Vincent-Salomon, A., Saliou, A., Houy, A., Milder, M., Lantz, O., Ychou, M., Denis, M. G., Pierga, J. Y., Stern, M. H., & Proudhon, C. (2018). Multiple Hotspot Mutations Scanning by Single Droplet Digital PCR. Clinical chemistry, 64(2), 317\u2013328. https://doi.org/10.1373/clinchem.2017.272518\n\n\nBortolini Silveira, A., Bidard, F. C., Tanguy, M. L., Girard, E., Tr\xe9dan, O., Dubot, C., Jacot, W., Goncalves, A., Debled, M., Levy, C., Ferrero, J. M., Jouannaud, C., Rios, M., Mouret-Reynier, M. A., Dalenc, F., Hego, C., Rampanou, A., Albaud, B., Baulande, S., Berger, F., \u2026 Pierga, J. Y. (2021). Multimodal liquid biopsy for early monitoring and outcome prediction of chemotherapy in metastatic breast cancer. NPJ breast cancer, 7(1), 115. https://doi.org/10.1038/s41523-021-00319-4\n\xa0\n\nRelated Episodes\n\nEpigenome-based Precision Medicine (Eleni Tomazou)\n\n\nEpigenetics and Epitranscriptomics in Cancer (Manel Esteller)\n\n\nDNA Methylation and Mammalian Development (D\xe9borah Bourc'his)\n\n\xa0\nContact\n\nEpigenetics Podcast on Twitter\n\n\nEpigenetics Podcast on Instagram\n\n\nEpigenetics Podcast on Mastodon\n\n\nActive Motif on Twitter\n\n\nActive Motif on LinkedIn\n\n\nEmail: podcast@activemotif.com