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In this episode of the Epigenetics Podcast, we caught up with Ben Delatte Research Scientist at Active Motif to talk about his work on Anchor Based Bisulfite Sequencing.\nWhole Genome Bisulfite Sequencing (WGBS) is the current standard for DNA methylation profiling. However, this approach is costly as it requires sequencing coverage over the entire genome. Here we introduce Anchor-Based Bisulfite Sequencing (ABBS). ABBS captures accurate DNA methylation information in Escherichia coli and mammals, while requiring up to 10 times fewer sequencing reads than WGBS. ABBS interrogates the entire genome and is not restricted to the CpG islands assayed by methods like Reduced Representation Bisulfite Sequencing (RRBS). The ABBS protocol is simple and can be performed in a single day.\n\xa0\nReferences\n\nChapin, N., Fernandez, J., Poole, J. et al. Anchor-based bisulfite sequencing determines genome-wide DNA methylation. Commun Biol 5, 596 (2022). https://doi.org/10.1038/s42003-022-03543-1\n\xa0\n\nRelated Episodes\n\nThe Role of DNA Methylation in Epilepsy (Katja Kobow)\n\n\nDNA Methylation and Mammalian Development (D\xe9borah Bourc'his)\n\n\nEffects of DNA Methylation on Diabetes (Charlotte Ling)\n\n\xa0\nContact\n\nActive Motif on Twitter\n\n\nEpigenetics Podcast on Twitter\n\n\nActive Motif on LinkedIn\n\n\nActive Motif on Facebook\n\n\nEmail: podcast@activemotif.com