Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.28.224816v1?rss=1 Authors: Renpeng, D., Liu, S., Chen, H., Kang, B., Drmanac, R., Gu, Y., Dong, X., Gao, Q. Abstract: PD-L1 signaling is important in regulating T cell function and keeping balance of tumor microenvironment, but its role in modifying TCR-T cell cytotoxicity remains unknown. MART-1-specific TCR-T cells (TCR-TMART-1) were stimulated by MEL-526 tumor cells expressing different proportions of PD-L1 and used to perform cytotoxicity assays and single-cell RNA sequencing. Percentage changes of different specific cell clusters were analyzed. The percentage of cluster HLA-DR+CD38+CD8+ was upregulated after antigen stimulation and tumor PD-L1 modified TCR-T cell function through downregulating the percentages of clusters HLA-DR+CD28+CD8+ and HLA-DR+CD38+CD8+ which were higher in TCR-TMART-1 than in Tnull. Copy rights belong to original authors. Visit the link for more info