Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.12.248021v1?rss=1 Authors: Noon, J. B., Sharma, A., Platten, J., Quinton, L. J., Reinhardt, C., Bosmann, M. Abstract: Interleukin-27 (IL-27) is a heterodimeric cytokine of the IL-12 family, formed by non-covalent association of the promiscuous EBI3 subunit and selective p28 subunit. IL-27 is produced by mononuclear phagocytes and unfolds pleiotropic immune-modulatory functions through high affinity ligation to IL-27 receptor alpha (IL-27RA). While IL-27 is known to contribute to immunity and to end inflammation following numerous types of infections, its relevance for host defense against multicellular parasites is still poorly defined. Here, we investigated the role of IL-27 during infection with the soil-transmitted hookworm, Nippostrongylus brasiliensis, in its early intrapulmonary life cycle. IL-27(p28) was detectable in broncho-alveolar lavage fluids of C57BL/6J wild type mice on day 1 after subcutaneous N. brasiliensis inoculation. The expression of IL-27RA was most abundant on lung invading {gamma}{delta} T cells followed by CD8+ T cells, CD4+ T cells and NK cells. IL-27RA was weakly present on CD19+ B cells and absent on neutrophils, alveolar macrophages and eosinophils. Il27ra-/- mice showed increased parasite burden together with aggravated pulmonary hemorrhage and higher alveolar albumin leakage as a surrogate for disruption of the epithelial/vascular barrier. Conversely, recombinant mouse IL-27 injections of wild type mice reduced parasite burdens and lung injury. In multiplex screens, we identified higher airway accumulations of IL-6, TNF and MCP-3 (CCL7) in Il27ra-/- mice, while rmIL-27 treatment showed a reciprocal effect. Finally, {gamma}{delta} T cell infiltration of the airways required endogenous IL-27 expression. In summary, this report demonstrates protective functions of IL-27 to control the early larval stage of hookworm infection in the lungs. Copy rights belong to original authors. Visit the link for more info