Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.16.207654v1?rss=1 Authors: Yoshioka, K., Nagahisa, H., Miura, F., Araki, H., Kamei, Y., Kitajima, Y., Seko, D., Nogami, J., Tsuchiya, Y., Okazaki, N., Yonekura, A., Ohba, S., Sumita, Y., Chiba, K., Ito, K., Asahina, I., Ogawa, Y., Ito, T., Ohkawa, Y., Ono, Y. Abstract: Skeletal muscle stem cells (satellite cells) are distributed throughout the body with heterogeneous properties that corresponds to region-specific pathophysiology. However, topographical genes that have functions remain unidentified in satellite cells of adult muscle. Here, we showed that expression of Homeobox (Hox)-A cluster genes, key regulators of the embryonic body plan, was robustly maintained in both muscles and satellite cells in adult mice and humans, which recapitulates their embryonic origin. We observed that regionally specific expressed Hox genes were linked to hypermethylation of the Hox-A locus. We examined Hoxa10 inactivation in satellite cells and found it led to genomic instability and mitotic catastrophe, which resulted in a decline in the regionally specific regenerative ability of muscles in adult mice. Thus, our results showed that Hox gene expression profiles instill the embryonic history in satellite cells as positional memory, potentially modulating the region-specificity in adult skeletal muscles. Copy rights belong to original authors. Visit the link for more info