Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.24.219147v1?rss=1 Authors: Yu, Q., Kilik, U., Holloway, E. M., Tsai, Y.-H., Wu, A., Wu, J. H., Czerwinski, M., Childs, C., He, Z., Glass, I. A., Higgins, P. D. R., Treutlein, B., Spence, J. R., Camp, J. G. Abstract: Human intestinal organoids (HIOs) generated from pluripotent stem cells provide extraordinary opportunities to explore development and disease. Here, we generate a single-cell transcriptome reference atlas from HIOs and from multiple developing human organs to quantify the specificity of HIO cell fate acquisition, and to explore alternative fates. We identify epithelium-mesenchyme interactions, transcriptional regulators involved in cell fate specification, and stem cell maturation features in the primary tissue that are recapitulated in HIOs. We use an HIO time course to reconstruct the molecular dynamics of intestinal stem cell emergence, as well as the specification of multiple mesenchyme subtypes. We find that the intestinal master regulator CDX2 correlates with distinct phases of epithelial and mesenchymal development, and CDX2 deletion perturbs the differentiation of both intestinal epithelium and mesenchyme. Collectively our data provides a comprehensive and quantitative assessment of HIO development, and illuminates the molecular machinery underlying endodermal and mesodermal cell fate specification. Copy rights belong to original authors. Visit the link for more info