Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.11.244939v1?rss=1 Authors: Raskar, T., Niebling, S., Devos, J. M., Yorke, B. A., Härtlein, M., Huse, N., Forsyth, T. V., Seydel, T., Pearson, A. R. Abstract: Incoherent neutron spectroscopy, in combination with dynamic light scattering was used to investigate the effect of ligand binding on the center-of-mass self-diffusion and internal diffusive dynamics of E.coli aspartate -decarboxylase (ADC). The X-ray crystal structure of the D-serine inhibitor complex with ADC was also determined, and molecular dynamics simulations used to further probe the structural rearrangements that occur as a result of ligand binding. These experiments reveal the existence of higher order oligomers of the ADC tetramer on ns-ms time-scales, and also show that ligand binding both affects the ADC internal diffusive dynamics and appears to further increase the size of the higher order oligomers. Copy rights belong to original authors. Visit the link for more info