Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.03.366427v1?rss=1 Authors: Liu, Y., Luo, C., Li, T., Zhang, W., Zong, Z., Liu, X., Deng, H. Abstract: In the present study, we developed a chemical method to produce dihydro nicotinamide mononucleotide (NMNH), which is the reduced-form of nicotinamide mononucleotide (NMN). We demonstrated that NMNH was a better NAD+ enhancer compared to NMN both in vitro and in vivo mediated by NMNAT. More importantly, NMNH significantly increased the reduced NAD (NADH) levels in cells and in mouse liver, resulting in cellular reductive stress and elevated reactive oxygen species. NMNH also induced cell cycle arrest and suppressed cell growth. Metabolomic analysis revealed that NMNH inhibited glycolysis and TCA cycle. However, NMNH treatment did not cause observable difference in mice. Taken together, our work demonstrates that NMNH is a potent NAD+ enhancer, and induces reductive stress to suppress cell growth. Copy rights belong to original authors. Visit the link for more info