Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.18.303248v1?rss=1 Authors: Rembeza, E., Engqvist, M. K. Abstract: Microfluidic platforms offer a drastic increase in throughput while minimizing sample usage and hands-on time, which makes them important tools for large-scale biological studies. A range of such systems have been developed for enzyme activity studies, although their complexity largely hinders their application by a wider scientific community. Here we present adaptation of an easy-to-use commercial microfluidic qPCR system for performing enzyme kinetics studies. We demonstrate functionality of the Fluidigm Biomark HD system (the Fluidigm system) by determining kinetic properties of three oxidases in a resorufin-based fluorescent assay. The results obtained in the microfluidic system proved reproducible and comparable to the ones obtained in a standard microplate-based assay. With a wide range of easy-to-use, off-the-shelf components, the microfluidic system presents itself as a simple and customizable platform for high-throughput enzyme activity studies. Copy rights belong to original authors. Visit the link for more info