Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.25.313395v1?rss=1 Authors: Xu, F., Shen, Y., Ding, L., Yang, C.-Y., Tan, H., Wang, H., Zhu, Q., Xu, R., Wu, F., Xu, C., Li, Q., Su, P., Zhang, L. I., Dong, H., Desimone, R., Xu, F., Hu, X., Lau, P.-M., Bi, G.-Q. Abstract: Whole-brain mesoscale mapping of primates has been hindered by large brain size and the relatively low throughput of available microscopy methods. Here, we present an integrative approach that combines primate-optimized tissue sectioning and clearing with ultrahigh-speed, large-scale, volumetric fluorescence microscopy, capable of completing whole-brain imaging of a rhesus monkey at 1 m x 1 m x 2.5 m voxel resolution within 100 hours. A progressive strategy is developed for high-efficiency, long-range tracing of individual axonal fibers through the dataset of hundreds of terabytes, establishing a "Serial sectioning and clearing, 3-dimensional Microscopy, with semi-Automated Reconstruction and Tracing" (SMART) pipeline. This system supports effective connectome-scale mapping of large primates that reveals distinct features of thalamocortical projections of the rhesus monkey brain at the level of individual axonal fibers. Copy rights belong to original authors. Visit the link for more info