Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.04.18.048397v1?rss=1 Authors: Zhang, S., Li, X., Drobizhev, M., Ai, H. Abstract: Serotonin (5-HT) is an important signaling monoamine in and beyond the central nervous system (CNS). We report structure-guided engineering of a green fluorescent, genetically encoded serotonin sensor (G-GESS) from a 5-HT-binding lipocalin in the soft tick. G-GESS shows fast response kinetics and high affinity, specificity, brightness, and photostability. We used G-GESS to image 5-HT dynamics in vitro and in behaving mice, demonstrating the broad utility of this novel biosensor. Copy rights belong to original authors. Visit the link for more info