Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.23.217992v1?rss=1 Authors: Furlan-Magaril, M., Ando-Kuri, M., Arzate-Mejia, R. G., Morf, J., Cairns, J., Poot- Hernandez, C. A., Andrews, S., Varnai, C., Virk, B., Wingett, S. W., Fraser, P. Abstract: Circadian gene expression is essential for organisms to adjust cellular responses and anticipate daily changes in the environment. In addition to its physiological importance, the clock circuit represents an ideal, temporally resolved, system to study transcription regulation. Here, we analyzed changes in spatial mouse liver chromatin conformation using genome-wide and promoter-capture Hi-C alongside daily oscillations in gene transcription in mouse liver. We found circadian topologically associated domains switched assignments to the transcriptionally active, open chromatin compartment and the inactive compartment at different hours of the day while their boundaries stably maintain their structure over time. Individual circadian gene promoters displayed maximal chromatin contacts at times of peak transcriptional output and the expression of circadian genes and contacted transcribed regulatory elements, or other circadian genes, was phase-coherent. Anchor sites of promoter chromatin loops were enriched in binding sites for liver nuclear receptors and transcription factors, some exclusively present in either rhythmic or stable contacts. The circadian 3D chromatin maps provided here identify the scales of chromatin conformation that parallel oscillatory gene expression and protein factors specifically associated with circadian or stable chromatin configurations. Copy rights belong to original authors. Visit the link for more info