Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.01.232496v1?rss=1 Authors: Liu, Y., Yang, M., Deng, Y., Su, G., Archibald Enninful, A., Guo, C. C., Tebaldi, T., Zhang, D., Kim, D., Bai, Z., Norris, E., Pan, A., Li, J., Xiao, Y., Halene, S., Fan, R. Abstract: We present DBiTseq Deterministic Barcoding in Tissue for spatial omics sequencing for comapping of mRNAs and proteins in a formaldehyde fixed tissue slide via NGS sequencing. Parallel microfluidic channels were used to deliver DNA barcodes to the surface of a tissue slide and crossflow of two sets of barcodes A1 to A50 and B1 to B50 followed by ligation in situ yielded a 2D mosaic of tissue pixels, each containing a unique full barcode AB. Application to mouse embryos revealed major tissue types in early organogenesis as well as fine features like microvasculature in a brain and pigmented epithelium in an eye field. Gene expression profiles in 10um pixels conformed into the clusters of single cell transcriptomes, allowing for rapid identification of cell types and spatial distributions. DBiT seq can be adopted by researchers with no experience in microfluidics and may find applications in a range of fields including developmental biology, cancer biology, neuroscience, and clinical pathology. Copy rights belong to original authors. Visit the link for more info