Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.24.219782v1?rss=1 Authors: Eberhardt, P., Slavov, C., SoĢrmann, J., Bamann, C., Braun, M., Wachtveitl, J. Abstract: Here we applied target analysis to a temperature dependent flash photolysis dataset of the light-driven sodium ion pump Krokinobacter rhodopsin 2 (KR2) at sodium pumping conditions. With an increase in temperature from 10 - 40 {degrees}C, the overall photocycle duration was accelerated by a factor of six, while single transitions like the L to M transition increased by a factor of 40. Using kinetic modeling with the Eyring constraint as well as spectral corrections on the datasets the spectral position as well as the equilibria of the different photointermediates could be resolved. The results provide further insight into KR2s photocycle and energetics. Copy rights belong to original authors. Visit the link for more info