Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.21.214445v1?rss=1 Authors: Losakul, R., Tobar, D. E., Pimenov, A., Gutierrez, A., Schipper, R., Jehle, W. A., Postma, H. W. C. Abstract: Nanopores are an established paradigm in genome sequencing technology, with remarkable advances still being made today. All efforts continually address the challenges associated with rapid, accurate, high-throughput, and low cost detection, particularly with long-read length DNA. We report on the in situ melting and unzipping of long, high molecular weight DNA. At varying salt concentration, we directly compare the translocation conductance and speeds between SiN and graphene nanopores at sub-10 nm pore diameters. We observe the force-induced unzipping of dsDNA at higher salt concentrations than previously reported in literature. We observe free running translocation without secondary structures of ssDNA that is an order of magnitude longer than reported before. We hypothesize that the frayed single strands at the molecules end get captured with a higher likelihood than both ends together. In understanding this phenomena for long-read lengths, we continue to address the challenges revolving around future generations of sequencing technology. Copy rights belong to original authors. Visit the link for more info