Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.12.243121v1?rss=1 Authors: Sharma, R., Pandey, N., Mongia, A., Mishra, S., Majumdar, A., KUMAR, V. Abstract: The advent of single-cell open-chromatin profiling technology has facilitated the analysis of heterogeneity of activity of regulatory regions at single-cell resolution. However, stochasticity and availability of low amount of relevant DNA cause high drop-out rate and noise in single-cell open-chromatin profiles. We introduce here a robust method called as Forest of Imputation Trees (FITs) to recover original signals from highly sparse and noisy single-cell open-chromatin profiles. FITs makes a forest of imputation trees to avoid bias during the restoration of read-count matrices. It resolves the challenging issue of recovering open chromatin signals without blurring out information at genomic sites with cell-type-specific activity. FITs is generalized for wider applicability, especially for highly sparse read-count matrices. The superiority of FITs in recovering signals of minority cells also makes it highly useful for single-cell open-chromatin profile from in vivo samples. Copy rights belong to original authors. Visit the link for more info