Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.21.299198v1?rss=1 Authors: Kim, K.-e., Park, I., Kim, J., Kang, M.-G., Choi, W. G., Shin, H., Kim, J.-S., Rhee, H.-W., Suh, J. M. Abstract: Here we describe iSLET (in situ Secretory protein Labeling via ER-anchored TurboID) which labels secretory pathway proteins as they transit through the ER-lumen to enable dynamic tracking of tissue-specific secreted proteomes in vivo. We expressed iSLET in the mouse liver and demonstrated efficient in situ labeling of the liver-specific secreted proteome which could be tracked and identified within circulating blood plasma. iSLET is a versatile and powerful tool for studying spatiotemporal dynamics of secretory proteins, a valuable class of biomarkers and therapeutic targets. Copy rights belong to original authors. Visit the link for more info