Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.24.220400v1?rss=1 Authors: Martis B, S., Droux, M., Deboudard, F., Nasser, W., Meyer, S., Reverchon, S. Abstract: A rapid and sensitive High Performance Liquid Chromatography (HPLC) method with UV and fluorescence detection is developed for routine analysis of 2-Keto-3-deoxy-gluconate (KDG), a catabolite product of pectin and alginate. These polysaccharides are both used for biofuel production and to generate high-value-added products. HPLC is performed, after derivatization of the carbonyl groups of the metabolite with O-phenylenediamine (OPD), using a linear gradient of trifluoroacetic acid and acetonitrile. Quantification is accomplished with an internal standard method. The gradient is optimized to distinguish KDG from its close structural analogues such as 5-keto-4-deoxyuronate (DKI) and 2,5-diketo-3-deoxygluconate (DKII). The proposed method is simple, highly sensitive and accurate for time course analysis of pectin or alginate degradation. Copy rights belong to original authors. Visit the link for more info