Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.08.287656v1?rss=1 Authors: Schachner, L. F., Jooss, K., Morgan, M. A., Piunti, A., Meiners, M. J., Lee, A., Kafader, J. O., Iwanaszko, M., Cheek, M. A., Burg, J. M., Howard, S. A., Keogh, M.-C., Shilatifard, A., Kelleher, N. L. Abstract: Nuc-MS characterizes histone modifications and variants directly from intact endogenous nucleosomes. Preserving whole nucleosome particles enables precise interrogation of their protein content, as for H3.3-containing nucleosomes which had 6-fold co-enrichment of variant H2A.Z over bulk chromatin. Nuc-MS, validated by ChIP-seq, showed co-occurrence of oncogenic H3.3K27M with euchromatic marks (e.g., H4K16ac and >15-fold enrichment of H3K79me2). By capturing the entire epigenetic landscape, Nuc-MS provides a new, quantitative readout of nucleosome-level biology. Copy rights belong to original authors. Visit the link for more info