Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.22.350488v1?rss=1 Authors: Schakowski, K. M., Elm, C., Linders, J., Kirsch, M. Abstract: In this work we present a method for the encapsulation of intracellular enzymes into a shell of albumin, using the example of catalase. We take advantage of organic, inorganic and physical chemistry for the preparation of biochemically active micrometer particles to prevent oxygen toxicity induced by artificial oxygen carriers of any type. In cell culture experiments, catalase capsules presented to be non-noxious in absence of peroxides. However, application of low and medium dosed capsules below 0.05 vol% (final concentration 0.001 vol%) increased the process of cell damaging induced by hydrogen peroxide and its decomposition products, while high dosed capsules > 0.05 vol% nullified all cell damage up to 5 mM hydrogen peroxide and reduced up to 90% of cellular damage for higher peroxide concentrations. The over-all activity of prepared catalase capsules was determined to be > 1000 U * mL-1 * vol%-1. Copy rights belong to original authors. Visit the link for more info