Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.22.308197v1?rss=1 Authors: van den Berg, B., Van den Eynde, R., Mueller, M., Vandenberg, W., Dedecker, P. Abstract: Maintaining focus during time-lapse experiments remains an important challenge in optical microscopy. In this paper we present an automated focusing system for fluorescence microscopy that can be used in time-lapse experiments. We provide software for flexibly operating the autofocus system and a complete alignment guide. The system is evaluated using a time-lapse experiment with temperature variations on Tetraspeck beads. Copy rights belong to original authors. Visit the link for more info