Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.20.260307v1?rss=1 Authors: Welsh, J. A., Killingswroth, B., Kepley, J., Traynor, T., McKinnon, K., Savage, J., Appel, D., Aldape, K., Camphausen, K., Berzofsky, J. A., Ivanov, A. R., Ghiran, I. H., Jones, J. C. Abstract: Evidence continues to increase of the clinical utility extracellular vesicles (EVs) can provide as translational biomarkers. While a wide variety of EV isolation and purification methods have been implemented, few techniques are high-throughput and scalable for removing excess fluorescent reagents (e.g. dyes, antibodies). EVs are too small to be recovered from routine cell-processing procedures, such as filtration or centrifugation. The lack of suitable methods for removing unbound labels, especially in optical assays, is a major roadblock to accurate EV phenotyping and utilization of EV assays in a translational or clinical setting. Therefore, we developed a method for using a multi-modal resin, referred to as EV-Clean, to remove unbound labels from EV samples, and we demonstrate improvement in flow cytometric EV analysis with the use of this EV-Clean method. Copy rights belong to original authors. Visit the link for more info