Rebecca Nance is a research assistant and PhD student in Dr. Bruce Smith's lab in the Scott-Ritchey Research Center at the Auburn University College of Veterinary Medicine in Auburn, Alabama. Rebecca is involved in modifying Canine Adenovirus Type 2 to explore targeted oncolytic virotherapy in dogs. Using a transcriptomic sequencing approach, the team aims to identify potential targets that are unique to cancerous tissue when compared to the corresponding tissue of origin. In this show, she discusses simple protocol modifications that allowed the isolation of moderately intact RNA from the dense, brittle, and hypocellular bone matrix of canine phalanges, with minimal contamination from connective tissue and marrow, in a quantity and quality suitable for transcriptomic sequencing. Referenced Links: A method for isolating RNA from canine bone, BioTechniques. https://doi.org/10.2144/btn-2019-0153 A method for isolating RNA from canine bone, protocols.io. dx.doi.org/10.17504/protocols.io.bf9vjr66