Evolution des programmierten Zelltods
The simple metazoan Hydra does posses two genes for caspases: Caspase 3A and caspase 3B. We generated antibodies against these caspases. By immunofluorescence staining and cell fractionation we demonstrated that both caspases are localized in mitochondria in vivo.Caspase 3A is a constitutively active enzyme independent of PCD. After induction of apoptosis caspase 3B translocates from the mitochondria to the cytoplasm and is activated. Activity of 3B is DEVD-specific.