BROADCASTS.com

Hosts:\xa0Vincent Racaniello,\xa0Dickson Despommier,\xa0and Daniel Griffin

The\xa0TWiPinella\xa0solve the case of the Woman from Guinea, and describe the use of CRISPR/Cas9 to identify essential apicomplexan genes.

• Malarias in\xa0Guinea\xa0(CDC)
• Genome-wide\xa0CRISPR screen\xa0in\xa0Toxoplasma\xa0(Cell)
• Plasmodium\xa0parasitemia associated with\xa0increased Ebola survival\xa0(Clin Inf Dis)
• Image credit
• Letters read\xa0on TWiP 118

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Little bit of a twist - a human family with eosinophilia. Conveyed by good friend/colleague ID physician. Australia, NSW, 45 yo Dad, having problem with mild abdominal distention. Seen by doc in Sydney, CBC shows eosinophil count of 10,500. Wife of same age reports feeling fine, but gets CBC and also shows eosinophils of 5,200. Two daughters, 17 and 19, no symptoms, bloods: 900 and normal eosinophils. One week prior to dads symptoms, sister in law came (also in NSW) and stayed, developed severe diarrhea, discomfort, bloating, weight loss, eosinohils 4,700. Eat raw fish (not known if fresh or salt) purchased at local markets. No overseas travel or out of urban environment. No pets, no home grown foods. Went back to previous labs and found normal eosinophil levels. Full workup for strongyloides, all negative. No HIV. No toxic habits, no remarkable medical history.

Send your case diagnosis, questions and comments to\xa0twip@microbe.tv