Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.29.179077v1?rss=1 Authors: Tong, L., Yuan, P., Chen, M., Chen, F., Bewersdorf, J., Zhou, J. Z., Grutzendler, J. Abstract: Optogenetics at single-cell resolution can be achieved by two-photon stimulation; however, this requires intense or holographic illumination. We markedly improve stimulation efficiency by positioning fluorophores with high two-photon cross-sections adjacent to opsins. The two-photon-excited fluorescence matches the opsin absorbance and can stimulate opsins in a highly localized manner through efficient single-photon absorption. This indirect fluorescence transfer illumination allows experiments difficult to implement in the live brain such as all-optical neural interrogation and control of regional cerebral blood flow. Copy rights belong to original authors. Visit the link for more info