Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.31.274100v1?rss=1 Authors: Rocha, M. D., Dreier, J., Brewer, J., Gahr, M., Vellema, M. Abstract: Sex hormones are essential modulators of birdsong. Testosterone, and its active androgenic and estrogenic metabolites, 5a-dihydrotestosterone (DHT) and estradiol, can re-shape the brain circuits responsible for song learning and production. The differential mechanisms of action of these different hormones during song development and song maintenance are, nonetheless, not fully understood. Here we demonstrate that unlike testosterone, DHT treatment does not induce singing behavior in naive adult female canaries that have never previously produced song. However, in birds with previous testosterone-induced singing experience, DHT alone is enough to promote the re-acquisition of high quality songs, even after months of silence. In addition, we show that the synaptic reorganization that accompanies vocal motor skill development requires more than DHT-induced androgen receptor activation. These results indicate that vocal motor practice will persistently modify the hormone-sensitive brain circuit responsible for song production, suggesting a mechanistic differentiation in the hormone-dependent regulation of the initial vocal motor skill acquisition and later re-acquisition. Copy rights belong to original authors. Visit the link for more info