Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.10.292201v1?rss=1 Authors: Segal, D., Valyear, M. D., Chaudhri, N. Abstract: The rats used in the current study were bred in our on-site TH::Cre rat breeding colony, in which 4 heterozygote TH::Cre positive dams were paired with 2 wild type sires (Charles River, St. Hubert, QC). Pups were weaned at twenty-three days post-natal, seprated by sex, and pair-housed in polycarbonate shoebox cages containing beta chip bedding (Aspen Sani chips; Envigo, Indianapolis, IN), a nylabone chew toy (Nylabones, Bio-Serv, Flemington, NJ), a red plastic tunnel (Rat retreats, Bio-Serv, Flemington, NJ), and shredded paper for enrichment, with unrestricted access to standard rat chow (Rodent Diet, Charles River, St. Hubert, QC) and water. Males and females were housed on separate walls within the breeding colony room held at 21 +/- 2 degrees celsius and approximately 40-50% humidity on a 12-hour light-dark cycle (lights on at 0700 hours). One week following weaning, two rounds of pinna samples were collected, and two rounds of genotyping were conducted to verify the genotype of each rat. Those that showed a negative genotype in both rounds (n = 31) were selected for the current study. Rats were transferred into the colony room at ~9 weeks of age and single housed (See Methods). Copy rights belong to original authors. Visit the link for more info