Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.12.147975v1?rss=1 Authors: Kavalali, E. T., Afuwape, O., Chanaday, N., Kasap, M., Monteggia, L. M. Abstract: Dynamins are GTPases required for pinching vesicles off the plasma membrane once a critical curvature is reached during endocytosis. Here, we probed dynamin function in central synapses by depleting all three dynamin isoforms in postnatal hippocampal neurons. We found a decrease in the propensity of evoked neurotransmission as well as a reduction in synaptic vesicle numbers. Using the fluorescent reporter vGluT1-pHluorin, we observed that compensatory endocytosis after 20 Hz stimulation was arrested in ~40% of presynaptic boutons, while remaining synapses showed only a modest effect suggesting the existence of a dynamin-independent endocytic pathway in central synapses. Surprisingly, we found that the retrieval of single synaptic vesicles, after either evoked or spontaneous fusion, was largely impervious to disruption of dynamins. Overall, our results suggest that classical dynamindependent endocytosis is not essential for retrieval of synaptic vesicle proteins after quantal single synaptic vesicle fusion. Copy rights belong to original authors. Visit the link for more info