Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.28.225011v1?rss=1 Authors: Costantini, I., Baria, E., Sorelli, M., Matuschke, F., Giardini, F., Menzel, M., Mazzamuto, G., Silvestri, L., Cicchi, R., Amunts, K., Axer, M., Pavone, F. S. Abstract: Analyzing the structure of neuronal fibers with single axon resolution, in large volumes, remains an unresolved challenge in connectomics. Here, we propose MAGIC (Myelin Autofluorescence imaging by Glycerol Induced Contrast enhancement), a simple tissue preparation method to perform label-free fluorescence imaging of myelinated fibers. We demonstrate its broad applicability by performing mesoscopic reconstruction at sub-micron resolution of mouse, rat, monkey, and human brain samples and by quantifying the different fiber organization in Control and Reeler mouse's hippocampal sections. Copy rights belong to original authors. Visit the link for more info