Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.14.251389v1?rss=1 Authors: Mirisis, A. A., Kopec, A. M., Carew, T. J. Abstract: Signaling through distinct growth factor pathways is required for long-term memory (LTM) in Aplysia. In a two-trial paradigm, Trial 1 TrkB signaling is required for expression of the immediate early gene apc/ebp, which is transient and necessary, but insufficient, for LTM formation. A prolonged increase in apc/ebp gene expression, which is both necessary and sufficient for LTM is achieved through TGF{beta} signaling during Trial 2. Here we demonstrate: (1) the prolonged increase in apc/ebp expression is not dependent on de novo transcription but is dependent on p38 MAPK activity induced by TGF{beta} ; (2) Trial 2 increases the interaction between apc/ebp mRNA and the stabilizing RNA-binding protein ApELAV; and (3) inhibition of the apc/ebp mRNA-ApELAV interaction blocks induction of LTM. These results reveal a novel role for post-transcriptional gene regulation: the stabilization of a specific transcript, apc/ebp, by ELAV-like proteins, as a critical molecular step in LTM formation. Copy rights belong to original authors. Visit the link for more info