Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.20.305508v1?rss=1 Authors: Yu, C.-H., Stirman, J. N., Yu, Y., Hira, R., Smith, S. L. Abstract: Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large Field-of-view Two-Photon imaging (Diesel2p). Combining novel optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view (~ 25 mm2) with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain. Copy rights belong to original authors. Visit the link for more info