Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.04.28.065680v1?rss=1 Authors: Kastli, R., Vighagen, R., van der Bourg, A., Argunsah, A. O., Iqbal, A., Voigt, F. F., Kirschenbaum, D., Aguzzi, A., Helmchen, F., Karayannis, T. Abstract: Two inhibitory cell types involved in modulating barrel cortex activity and perception during active whisking in adult mice, are the VIP+ and SST+ interneurons. Here we identify a developmental transition point of structural and functional rearrangements onto these interneuron types around the start of active sensation at P14. Using in vivo two-photon Ca2+ imaging, we find that before P14, both interneuron types respond stronger to a multi-whisker stimulus, whereas after P14 their responses diverge, with VIP+ cells losing their multi-whisker preference and SST+ neurons enhancing theirs. Rabies virus tracings followed by tissue clearing, as well as photostimulation-coupled electrophysiology reveal that SST+ cells receive higher cross-barrel inputs compared to VIP+ at both time points. In addition, we also uncover that whereas prior to P14 both cell types receive direct input from the sensory thalamus, after P14 VIP+ cells show reduced inputs and SST+ cells largely shift to motor-related thalamic nuclei. Copy rights belong to original authors. Visit the link for more info