Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.05.20.105858v1?rss=1 Authors: Uggerud, I. M., Krakenes, T., Hirai, H., Vedeler, C. A., Schubert, M. Abstract: Improved understanding of the mechanisms involved in neurodegenerative disease has been hampered by the lack of robust cellular models that faithfully replicate in vivo features. Here, we present a refined protocol for generating age-dependent, well-developed and synaptically active rat Purkinje neurons, responsive to paracrine factors and supporting a 3D cell network. Our model provides high experimental flexibility, high-throughput screening capabilities and reliability to elucidate Purkinje neuron function, communication and neurodegenerative mechanisms. Copy rights belong to original authors. Visit the link for more info