Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.05.237537v1?rss=1 Authors: Fearns, A., Greenwood, D., Rodgers, A., Jiang, H., Gutierrez, M. Abstract: Correlative light, electron and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed CLEIM in tissue (CLEIMiT), and used it to identify the cell-type specific accumulation of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis. Using CLEIMiT, we found that the anti-TB drug bedaquiline is localised not only in foamy macrophages in the lungs during infection but also accumulate in polymorphonuclear (PMN) cells. Copy rights belong to original authors. Visit the link for more info