Empirically tracking the exposure of human plasma & serum samples to thawed conditions
Exposure of blood plasma/serum (P/S) to thawed conditions (> -30 \xb0C) can produce biomolecular changes that skew measurements of biomarkers within archived patient samples, potentially rendering them unfit for molecular analysis. Since freeze-thaw histories are often poorly documented, objective methods for assessing molecular fitness prior to analysis are needed.