Separation and Purification Techniques

Published: May 9, 2024, 8 a.m.

This episode of MCAT Basics covers several separation and purification techniques you may see on the MCAT. We\u2019ll cover these techniques in three distinct categories: small molecules, proteins, and cells.\xa0

Within the small molecule category, we\u2019ll discuss extraction, crystallization and recrystallization, and chromatography (TLC and HPLC).

For proteins, we cover SDS-page, precipitation, soelectric focusing, and chromatography variations. Lastly, we\u2019ll explain, within the world of cells, centrifugation, flow cytometry, and microfluidics.\xa0

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Visit MedSchoolCoach.com for more help with the MCAT.\xa0

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Jump into the conversation:

[00:00] MCAT Tutorig from MedSchoolCoach

[00:34] Welcome to MCAT Basics

[01:07] Topics covered in this episode

[02:24] Techniques for small molecules - extraction, crystallization and recrystallization, and chromatography (TLC and HPLC)

[28:24] Techniques for proteins - SDS-page, precipitation, isoelectric focusing, and chromatography variations

[54:47] Techniques for cells - centrifugation, flow cytometry, and microfluidics.\xa0

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